Amano T, Richelson E, Nirenberg M
Proc Natl Acad Sci U S A. 1972 Jan;69(1):258-63. doi: 10.1073/pnas.69.1.258.
Neuroblastoma clones were examined for choline acetyltransferase (EC 2.3.1.6), tyrosine hydroxylase (EC 1.14.3.a), acetylcholinesterase (EC 3.1.1.7), and also for neurite formation. One clone does not form axons or dendrites. Three types of clones were found with respect to neurotransmitter synthesis: cholinergic, adrenergic, and clones that do not synthesize acetylcholine or catechols. All clones contain acetylcholinesterase. These results show that genes determining neurotransmitter species can be expressed in dividing cells, that the parental programs of gene expression are inherited, and that dividing cells can be programmed with respect to their ability to communicate with other cells.
对神经母细胞瘤克隆进行了胆碱乙酰转移酶（EC 2.3.1.6）、酪氨酸羟化酶（EC 1.14.3.a）、乙酰胆碱酯酶（EC 3.1.1.7）的检测，同时也检测了神经突的形成。一个克隆不形成轴突或树突。在神经递质合成方面发现了三种类型的克隆：胆碱能型、肾上腺素能型以及不合成乙酰胆碱或儿茶酚胺的克隆。所有克隆都含有乙酰胆碱酯酶。这些结果表明，决定神经递质种类的基因可以在分裂细胞中表达，基因表达的亲本程序是可遗传的，并且分裂细胞在与其他细胞通讯的能力方面可以被编程。
