Steinitz M, Klein G
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3518-20. doi: 10.1073/pnas.72.9.3518.
The GC-BJAB cell line, which carries the Epstein--Barr virus (EBV), was derived from an EBV-genome-negative lymphoma line (BJAB) by EBV infection in vitro [G. B. Clements, G. Klein, and S. Povey (1975) Int. J. Cancer, in press]. Both lines grow at a similar rate at 37 degrees but they differ at other temperatures. BJAB grows well at 34 degrees, 37 degrees, and 39 degrees. GC-BJAB grows at 37 degrees and 39 degrees, but grows poorly at 34 degrees. At 37 degrees, GC-BJAB cultures can be maintained at the viable state for a long time after having reached saturation density (approximately 10(6) cells per ml). In contrast, BJAB cultures die very soon after having attained similar maximum density. Since the identity of the two cell lines has been critically established [Clements et al.; E. Svedmyr and M. Jondal (1975) Proc. Nat. Acad. Sci USA 72, 1622--1626; G. Klein, to be published; J. Zeuthen, personal communication] the remarkable differences in their growth properties must be attributed to the EBV genome.
携带爱泼斯坦-巴尔病毒（EBV）的GC-BJAB细胞系，是通过体外EBV感染，从一个EBV基因组阴性的淋巴瘤细胞系（BJAB）衍生而来的[G. B. 克莱门茨、G. 克莱因和S. 波维（1975年），《国际癌症杂志》，即将发表]。这两个细胞系在37摄氏度时生长速率相似，但在其他温度下有所不同。BJAB在34摄氏度、37摄氏度和39摄氏度时生长良好。GC-BJAB在37摄氏度和39摄氏度时生长，但在34摄氏度时生长不良。在37摄氏度时，GC-BJAB培养物在达到饱和密度（约每毫升10(6)个细胞）后可长时间维持在存活状态。相比之下，BJAB培养物在达到相似的最大密度后很快死亡。由于这两个细胞系的身份已得到严格确定[克莱门茨等人；E. 斯韦德米尔和M. 琼达尔（1975年），《美国国家科学院院刊》72, 1622 - 1626；G. 克莱因，待发表；J. 措滕，个人交流]，它们生长特性的显著差异必定归因于EBV基因组。
