Gautam A M, Glynn P
Multiple Sclerosis Society Laboratory, Institute of Neurology, London, U.K.
J Neuroimmunol. 1991 Oct;34(1):25-31. doi: 10.1016/0165-5728(91)90095-o.
Splenic T cells from myelin basic protein (MBP)-immunised Lewis rats were activated to transfer experimental autoimmune encephalomyelitis (EAE) by co-culture with MBP-pulsed lymphoid dendritic cells (DC). MBP-pulsed DC could be kept for at least 24 h at 37 degrees C in antigen-free medium without affecting their ability subsequently to activate encephalitogenic T cells. However, MBP-pulsed DC were rendered much less stimulatory after a 6 h, but not 2 h, secondary incubation with ovalbumin. Thus, although encephalitogenic complexes between MBP and DC appear very stable in the absence of competing antigens, in their presence, antigen exchange can take place over a period of a few hours; this has positive implications for therapy of EAE by antigen competition.
将来自用髓鞘碱性蛋白（MBP）免疫的Lewis大鼠的脾T细胞与用MBP脉冲处理的淋巴样树突状细胞（DC）共培养，可激活这些T细胞以转移实验性自身免疫性脑脊髓炎（EAE）。用MBP脉冲处理的DC在无抗原培养基中于37℃可保存至少24小时，而不影响其随后激活致脑炎T细胞的能力。然而，在用卵清蛋白进行6小时（而非2小时）的二次孵育后，用MBP脉冲处理的DC的刺激作用大大减弱。因此，尽管在没有竞争性抗原的情况下，MBP与DC之间的致脑炎复合物似乎非常稳定，但在有竞争性抗原存在时，抗原交换可在数小时内发生；这对抗原竞争治疗EAE具有积极意义。
