Gautam A M, Glynn P
Multiple Sclerosis Society Laboratory, Institute of Neurology, London, U.K.
J Neuroimmunol. 1989 Apr;22(2):113-21. doi: 10.1016/0165-5728(89)90041-6.
Lymphocytes isolated from Lewis rats immunised with protein antigen in adjuvant were stimulated to proliferate in vitro by splenic dendritic cells (DC) which had been pulsed with purified homologous myelin basic protein (MBP). By contrast, in parallel experiments, lymphocytes did not respond to ovalbumin unless the protein was first processed by macrophages by a chloroquine-sensitive mechanism. DC, pulsed with rat MBP at concentrations as low as 6 micrograms/ml, activated lymphocytes for transfer of severe experimental autoimmune encephalomyelitis (EAE). MBP dissociating from myelin membranes in physiological medium, as well as MBP purified from highly acidic extracts of myelin, was effective for pulsing DC; preincubating myelin with macrophages led to a reduction rather than an enhancement in the severity of the EAE transferred. It is concluded that macrophage-mediated antigen processing is not required for immunogenic presentation of the determinants of MBP which cause EAE in Lewis rats. Furthermore, MBP-pulsed DC may prove useful in experiments requiring activation of encephalitogenic T cells.
从用佐剂中的蛋白质抗原免疫的Lewis大鼠分离的淋巴细胞，被用纯化的同源髓鞘碱性蛋白（MBP）脉冲处理过的脾树突状细胞（DC）刺激在体外增殖。相比之下，在平行实验中，淋巴细胞对卵清蛋白没有反应，除非该蛋白质首先通过对氯喹敏感的机制被巨噬细胞处理。以低至6微克/毫升的浓度用大鼠MBP脉冲处理的DC，激活淋巴细胞以转移严重的实验性自身免疫性脑脊髓炎（EAE）。在生理介质中从髓鞘膜解离的MBP以及从髓鞘的高酸性提取物中纯化的MBP，对脉冲处理DC有效；用巨噬细胞预孵育髓鞘导致转移的EAE严重程度降低而非增强。得出的结论是，Lewis大鼠中引起EAE的MBP决定簇的免疫原性呈递不需要巨噬细胞介导的抗原处理。此外，MBP脉冲处理的DC可能在需要激活致脑炎性T细胞的实验中证明是有用的。
