Pedrazza Eduardo Luiz, Riboldi Gustavo Pelicioli, Pereira Grace Schenatto, Izquierdo Iván, Bonan Carla Denise
Laboratório de Neuroquímica e Psicofarmacologia, Departamento de Biologia Celular e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul. Avenida Ipiranga, 6681, 90619-900 Porto Alegre, RS, Brazil.
Neurosci Lett. 2007 Feb 8;413(1):21-4. doi: 10.1016/j.neulet.2006.11.023. Epub 2006 Dec 12.
ATP and adenosine may play a role in the mechanisms of synaptic plasticity and memory formation. Previous studies have shown that ecto-nucleotidase activities are altered during memory consolidation of an aversive task named step-down inhibitory avoidance. Here we investigate ecto-nucleotidase activities in hippocampal synaptosomes of rats submitted to training and test sessions of habituation to open field, which is one of the most elementary forms of learning. There were no significant alterations on ATP, ADP and AMP hydrolysis immediately after the training session. However, immediately after the test session (0min), there was a significant increase of ATP hydrolysis (61%), but not of ADP and AMP hydrolysis. Sixty minutes after the test session, a significant increase of NTPDase (75% and 60.5% for ATP and ADP hydrolysis, respectively) and ecto-5'-nucleotidase (40%) activities was observed. This study reveals the involvement of ecto-nucleotidase activities in different learning paradigms during memory processing.
三磷酸腺苷（ATP）和腺苷可能在突触可塑性及记忆形成机制中发挥作用。先前的研究表明，在一种名为阶梯式抑制性回避的厌恶任务的记忆巩固过程中，胞外核苷酸酶的活性会发生改变。在此，我们研究了经历旷场适应训练和测试环节的大鼠海马突触体中的胞外核苷酸酶活性，旷场适应是最基本的学习形式之一。训练环节结束后，ATP、二磷酸腺苷（ADP）和一磷酸腺苷（AMP）的水解没有显著变化。然而，测试环节结束后立即（0分钟），ATP水解显著增加（61%），但ADP和AMP水解没有增加。测试环节结束60分钟后，观察到NTPDase（ATP和ADP水解分别增加75%和60.5%）和胞外5'-核苷酸酶（增加40%）的活性显著增加。这项研究揭示了胞外核苷酸酶活性在记忆处理过程中参与不同的学习范式。
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