Unidade de Experimentação Animal, Hospital de Clínicas de Porto Alegre, CEP 90035-903, Porto Alegre, RS, Brazil.
Brain Res. 2010 Feb 26;1316:173-80. doi: 10.1016/j.brainres.2009.11.076. Epub 2009 Dec 5.
Regular and moderate exercise has been considered as an interesting neuroprotective strategy. However, the molecular mechanisms by which physical exercise alters brain function are unclear. Purinergic signaling seems to modulate the pathophysiology of ischemic neuronal damage, since it has been described a neuroprotective activity of adenosine and a dual role of ATP. In the present study, we investigated the effect of daily moderate intensity exercise on ectonucleotidase activities in synaptosomes from hippocampus and the soluble nucleotidases from blood serum of rats. Adult male Wistar rats were assigned to non-exercised (sedentary) group and exercised during 20-min sessions on different programs. The effects of physical activity on hydrolysis of ATP, ADP and AMP were assayed in the synaptosomal fraction obtained from the hippocampus and serum approximately 16 h after the last training session. Our data demonstrated that a neuroprotective exercise protocol, daily 20 min of training in treadmill during 2 weeks, diminished significantly the ADP hydrolysis and there is a trend to reduce the ATP hydrolysis in both hippocampal synaptosomes and blood serum of rats. We suggest that the neuroprotective exercise protocol may modulate nucleotidase activities.
定期和适度的运动被认为是一种有趣的神经保护策略。然而，运动改变大脑功能的分子机制尚不清楚。嘌呤能信号似乎调节缺血性神经元损伤的病理生理学，因为已经描述了腺苷的神经保护活性和 ATP 的双重作用。在本研究中，我们研究了每日适度强度运动对海马突触体中核苷酸酶活性和大鼠血清中可溶性核苷酸酶的影响。成年雄性 Wistar 大鼠被分为不运动（静坐）组和在不同方案下进行 20 分钟的运动组。大约在最后一次训练结束后 16 小时，在从海马体获得的突触体部分和血清中测定了对 ATP、ADP 和 AMP 水解的影响。我们的数据表明，神经保护运动方案，每天在跑步机上训练 20 分钟，持续 2 周，显著降低了 ADP 的水解，并且在大鼠海马突触体和血清中，ATP 的水解也有降低的趋势。我们认为，神经保护运动方案可能调节核苷酸酶的活性。
