Bonan C D, Dias M M, Battastini A M, Dias R D, Sarkis J J
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
Neurochem Res. 1998 Jul;23(7):977-82. doi: 10.1023/a:1021084422228.
Several lines of evidence indicate that ATP may play an important role in Long-Term Potentiation. In this investigation we evaluated the effect of a memory task (step-down inhibitory avoidance) on the synaptosomal ecto-enzymes (ATP diphosphohydrolase and 5'-nucleotidase) involved in the degradation of ATP to adenosine. After the training session, a decrease in the ATPase (40%) and ADPase (29%) activities of ATP diphosphohydrolase as well as was a decrease in 5'-nucleotidase activity (31%) was observed in hippocampal synaptosomes of rats trained and killed immediately after training. In synaptosomes of rats killed 30 minutes after training, a decrease in ATPase activity (28%) was observed. In the test session, no significant changes were observed in the enzyme activities studied. These results provide new information about the activity of ecto-enzymes involved in nucleotide degradation and their possible participation in mechanisms of acquisition and modulation of memory processing.
多条证据表明，ATP可能在长时程增强中发挥重要作用。在本研究中，我们评估了记忆任务（跳台抑制性回避）对参与ATP降解为腺苷的突触体胞外酶（ATP二磷酸水解酶和5'-核苷酸酶）的影响。训练后，在训练后立即处死的训练大鼠的海马突触体中，观察到ATP二磷酸水解酶的ATP酶活性（40%）和ADP酶活性（29%）降低，同时5'-核苷酸酶活性也降低（31%）。在训练后30分钟处死的大鼠的突触体中，观察到ATP酶活性降低（28%）。在测试阶段，所研究的酶活性没有观察到显著变化。这些结果提供了关于参与核苷酸降解的胞外酶活性及其可能参与记忆处理的获取和调节机制的新信息。
