Rice Kevin M, Preston Deborah L, Neff David, Norton Michael, Blough Eric R
Laboratory of Molecular Physiology, Suite 311, Science Building, Department of Biological Sciences, 1 John Marshall Drive, Marshall University, Huntington, WV 25755-1090, USA.
J Gerontol A Biol Sci Med Sci. 2006 Nov;61(11):1119-29. doi: 10.1093/gerona/61.11.1119.
This study tested the hypothesis that age-related changes in the dystrophin-glycoprotein complex (DGC) may precede age-associated alterations in muscle morphology and function. Compared to those in adult (6 month) rats, extensor digitorum longus (EDL) and soleus muscle mass was decreased in old (30 month) and very old (36 month) Fischer 344/NNiaHSD x Brown Norway/BiNia rats. The amount of dystrophin, beta-dystroglycan, and alpha-sarcoglycan increased with aging in the EDL and decreased with aging in the soleus. alpha-Dystroglycan levels were increased with aging in both muscles and displayed evidence of altered glycosylation. Immunostaining for the presence of antibody infiltration and dystrophin following increased muscle stretch suggested that the aging in the soleus was characterized by diminished membrane integrity. Together, these data suggest that aging is associated with alterations in EDL and soleus DGC protein content and localization. These results may implicate the DGC as playing a role in age-associated skeletal muscle remodeling.
肌营养不良蛋白 - 糖蛋白复合物（DGC）与年龄相关的变化可能先于肌肉形态和功能与年龄相关的改变。与成年（6个月）大鼠相比，老年（30个月）和极老年（36个月）的Fischer 344/NNiaHSD×Brown Norway/BiNia大鼠的趾长伸肌（EDL）和比目鱼肌质量下降。肌营养不良蛋白、β - 肌营养不良聚糖和α - 肌聚糖的量在EDL中随衰老而增加，在比目鱼肌中随衰老而减少。α - 肌营养不良聚糖水平在两块肌肉中均随衰老而增加，并显示出糖基化改变的迹象。增加肌肉拉伸后，针对抗体浸润和肌营养不良蛋白存在情况的免疫染色表明，比目鱼肌衰老的特征是膜完整性降低。总之，这些数据表明衰老与EDL和比目鱼肌DGC蛋白含量及定位的改变有关。这些结果可能意味着DGC在与年龄相关的骨骼肌重塑中发挥作用。
