Pospelova T V, Medvedev A V, Kisliakova T V, Svetlikova S B, Pospelov V A
Mol Biol (Mosk). 1991 Jan-Feb;25(1):105-15.
Regulation of c-fos protooncogene activity in rat embryonal fibroblasts (REF), E1Aad5-immortalized REF cells, and E1Aad5 + cHa-ras transformed REF cells has been investigated. The analysis of regulation of fos-promoter activity was done by means of transient and stable transfection of fos-CAT plasmid into immortalized and transformed cells. In parallel, the regulation of cellular c-fos as well as c-jun and c-myc genes expression has been studied. It has been found that in E1Aad5 + cHa-ras-transformed cells the expression of c-fos promoter has a constitutive, non-inducible character while in REF cells and cells immortalized by E1Aad5 the fos-promoter can be regulated by serum growth factors, EGF, and TPA.
对大鼠胚胎成纤维细胞（REF）、E1Aad5永生化REF细胞以及E1Aad5 + cHa-ras转化REF细胞中c-fos原癌基因活性的调控进行了研究。通过将fos-CAT质粒瞬时和稳定转染到永生化和转化细胞中，对fos启动子活性的调控进行了分析。同时，研究了细胞c-fos以及c-jun和c-myc基因表达的调控。已发现，在E1Aad5 + cHa-ras转化细胞中，c-fos启动子的表达具有组成性、不可诱导的特征，而在REF细胞和由E1Aad5永生化的细胞中，fos启动子可受血清生长因子、表皮生长因子（EGF）和佛波酯（TPA）调控。
