Réthelyi M, Mohapatra N K, Metz C B, Petrusz P, Lund P K
Department of Physiology, University of North Carolina, Chapel Hill.
Neuroscience. 1991;42(2):531-9. doi: 10.1016/0306-4522(91)90395-5.
Hybridization signals indicating mRNAs encoding the precursor of calcitonin gene-related peptide (CGRP) and CGRP immunoreactivity were detected on parallel sections containing brainstem motor nuclei using in situ hybridization histochemistry and immunohistochemistry. In untreated and saline-injected rats the motoneurons in the hypoglossal, facial motor nuclei and in the ambiguus nucleus showed weak to moderate hybridization signals. In these motoneurons CGRP immunoreactivity was restricted to the Nissl bodies of the perikarya. Twenty-four and 42 hours after intracerebroventricular colchicine injection the intensity of both the hybridization signal and the immunoreaction product increased. The distribution of CGRP immunoreactivity changed from discrete perikaryal localization to diffuse reaction in the perikarya and along the proximal dendritic tree. Motoneurons in the rest of the brainstem motor nuclei (VIth, Vth, IVth and IIIrd) of untreated and saline-injected rats showed neither hybridization signal nor CGRP immunoreactivity. After intracerebroventricular injection of colchicine these motoneurons showed both hybridization signal and CGRP immunoreactivity. In all nuclei the size of motoneurons decreased and their Nissl structure changed to an amorphous basophilic mass following colchicine treatment.
采用原位杂交组织化学和免疫组织化学方法，在含有脑干运动核的平行切片上检测到了编码降钙素基因相关肽（CGRP）前体的mRNA的杂交信号及CGRP免疫反应性。在未处理和注射生理盐水的大鼠中，舌下神经核、面神经运动核和疑核中的运动神经元显示出弱至中度的杂交信号。在这些运动神经元中，CGRP免疫反应性局限于核周体的尼氏体。脑室内注射秋水仙碱24小时和42小时后，杂交信号强度和免疫反应产物强度均增加。CGRP免疫反应性的分布从离散的核周定位变为核周体和近端树突上的弥漫反应。未处理和注射生理盐水的大鼠脑干其余运动核（动眼神经核、三叉神经运动核、滑车神经核和展神经核）中的运动神经元既未显示杂交信号，也未显示CGRP免疫反应性。脑室内注射秋水仙碱后，这些运动神经元同时显示出杂交信号和CGRP免疫反应性。在所有核中，秋水仙碱处理后运动神经元的大小减小，其尼氏体结构变为无定形嗜碱性团块。
