Cortés R, Arvidsson U, Schalling M, Ceccatelli S, Hökfelt T
Department of Histology, Karolinska Institute, Stockholm, Sweden.
J Chem Neuroanat. 1990 Nov-Dec;3(6):467-85.
In situ hybridization techniques were used to analyse the distribution of cholecystokinin (CCK) mRNA in the lower brain stem, spinal cord and dorsal root ganglia of the rat and guinea pig, in comparison with that of mRNAs for calcitonin gene-related peptide (CGRP) and choline acetyltransferase. In the rat, CCK mRNA was found in numerous motoneurons in the spinal cord as well as in the motor trigeminal, facial and hypoglossal nuclei. Coexistence of CCK mRNA and CGRP mRNA could be established in spinal and brain stem motoneurons. Conversely, in the guinea pig CCK mRNA could only be detected in few motoneurons in the spinal cord. In both species, CCK mRNA was present in the spinal trigeminal nucleus and in the dorsal horn of the spinal cord, in numerous small cells located in the outer laminae (mainly II-IV), and in the rat was also found in large cells in laminae IV and V. Few small cells in laminae VI-VIII and X of the spinal cord and cells in several brain stem nuclei, such as the solitary tract, gracile and cuneate nuclei, also showed CCK mRNA in the rat. In the guinea pig brain stem CCK mRNA was found, among others, in the solitary tract nucleus, pontine reticular formation and pontine periventricular grey. In dorsal root ganglia CCK mRNA was abundant in the guinea pig, but almost absent in the rat, where only single cells were found that expressed low levels of this mRNA.
采用原位杂交技术分析大鼠和豚鼠延髓下部、脊髓及背根神经节中胆囊收缩素（CCK）mRNA的分布，并与降钙素基因相关肽（CGRP）和胆碱乙酰转移酶的mRNA分布进行比较。在大鼠中，CCK mRNA存在于脊髓中的众多运动神经元以及三叉神经运动核、面神经核和舌下神经核中。在脊髓和脑干运动神经元中可证实CCK mRNA和CGRP mRNA共存。相反，在豚鼠中，仅在脊髓中的少数运动神经元中检测到CCK mRNA。在这两个物种中，CCK mRNA均存在于三叉神经脊束核和脊髓背角，存在于位于外侧板层（主要为II-IV层）的众多小细胞中，在大鼠中还发现存在于IV层和V层的大细胞中。在大鼠脊髓VI-VIII层和X层中的少数小细胞以及几个脑干核团（如孤束核、薄束核和楔束核）中的细胞也显示有CCK mRNA。在豚鼠脑干中，除其他部位外，在孤束核、脑桥网状结构和脑桥室周灰质中发现有CCK mRNA。在背根神经节中，CCK mRNA在豚鼠中丰富，但在大鼠中几乎不存在，在大鼠中仅发现单个细胞表达低水平的这种mRNA。
