Xuan X N, Limcumpao J A, Horimoto T, Tohya Y, Azetaka M, Takahashi E, Mikami T
Department of Veterinary, Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Vet Microbiol. 1991 Aug 15;28(3):257-67. doi: 10.1016/0378-1135(91)90080-y.
Anti-idiotypic antibodies (anti-Id Abs) were produced in rabbits after inoculation with two mouse monoclonal antibodies (mAbs) directed against canine herpesvirus (CHV) glycoproteins (gps). One of the mAbs, 12H11, was directed against an epitope on gp 145/112 of CHV which induced virus neutralizing (VN) antibodies and against a cross-reacting epitope on the gp 143/108 of feline herpes-virus type 1 (FHV-1). The other mAb, 11F7, was directed against epitopes on CHV gp47 which induce VN and hemagglutination-inhibition (HAI) antibodies. Using VN-inhibition and HAI-inhibition assays with CHV and FHV-1, the anti-Id Abs obviously inhibited the activities of autologous mAbs, suggesting that anti-Id Abs mimic the epitopes of CHV gp 145/112 or FHV-1 gp 143/108 and CHV gp47 by binding the anti-combining site of the mAbs. These anti-Id Abs, when injected into mice, elicited specific CHV-neutralizing and HAI antibody responses, and one of them also elicited a specific FHV-1-neutralizing antibody response. These data supported the idea that immunization with anti-Id Ab can induce specific VN antibody response, as has been theorized by other workers.
用两种针对犬疱疹病毒（CHV）糖蛋白（gp）的小鼠单克隆抗体（mAb）接种兔子后，产生了抗独特型抗体（抗Id抗体）。其中一种mAb，即12H11，针对CHV gp 145/112上的一个表位，该表位可诱导病毒中和（VN）抗体，并且还针对1型猫疱疹病毒（FHV-1）gp 143/108上的一个交叉反应表位。另一种mAb，即11F7，针对CHV gp47上可诱导VN和血凝抑制（HAI）抗体的表位。通过对CHV和FHV-1进行VN抑制和HAI抑制试验，抗Id抗体明显抑制了同源mAb的活性，这表明抗Id抗体通过结合mAb的抗结合位点来模拟CHV gp 145/112或FHV-1 gp 143/108以及CHV gp47的表位。将这些抗Id抗体注射到小鼠体内时，可引发特异性的CHV中和及HAI抗体反应，其中一种还引发了特异性的FHV-1中和抗体反应。这些数据支持了用抗Id抗体免疫可诱导特异性VN抗体反应这一观点，其他研究人员也有过相关理论推测。
