Osterhaus A D, Rimmelzwaan G F, Weijer K, Uytdehaag A G
National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Viral Immunol. 1989 Winter;2(4):255-62. doi: 10.1089/vim.1989.2.255.
Syngeneic monoclonal anti-idiotypic antibodies were raised against idiotopes on neutralizing monoclonal antibodies with specificity for feline leukemia virus and canine parvovirus. The anti-idiotypic antibodies were shown to recognize paratope-related private idiotopes. Mice were injected with the monoclonal anti-idiotypic antibodies and the sera of these mice were screened for antiviral reactivities. Antibodies to both feline leukemia and canine parvovirus could be induced as determined by ELISA. These results suggest that anti-idiotypic antibodies which detect private idiotopes and thus do not represent internal images of viral antigens may be considered as candidates for the induction of antiviral immunity.
制备了同基因单克隆抗独特型抗体，该抗体针对对猫白血病病毒和犬细小病毒具有特异性的中和单克隆抗体上的独特型表位。结果表明，这些抗独特型抗体可识别与互补位相关的个体独特型表位。给小鼠注射单克隆抗独特型抗体，并筛选这些小鼠血清的抗病毒反应性。通过酶联免疫吸附测定法（ELISA）确定，可诱导出针对猫白血病病毒和犬细小病毒的抗体。这些结果表明，能够检测个体独特型表位、因而不代表病毒抗原内影像的抗独特型抗体，可被视为诱导抗病毒免疫的候选物。
