Rebrikov D V, Stepanova E V, Koroleva O V, Budarina Zh I, Zakharova M V, Iurkova T V, Solonin A S, Belova O V, Pozhidaeva Z A, Leont'evskiĭ A A
Prikl Biokhim Mikrobiol. 2006 Nov-Dec;42(6):645-53.
The main physicochemical characteristics of the major isoform of the laccase secreted by the fungu, Trametes hirsuta 072 were studied. The enzyme belongs to the group of high redox potential laccases (E(T1) = 790 +/- 5), and it oxidizes with high efficiency various substrates of phenolic nature. The gene of this isoform was cloned, and its nucleotide sequence was determined. The length of the complete gene is 2134 bp. It comprises 11 exons and 10 introns. Analysis of the amino acid sequence of T. hirsuta 072 laccase demonstrated a high homology (to 96.9%) to the other laccases secreted by fungi of the genus Trametes.
对真菌糙皮侧耳072分泌的漆酶主要同工型的主要物理化学特性进行了研究。该酶属于高氧化还原电位漆酶组（E(T1)=790±5），能高效氧化各种酚类性质的底物。克隆了该同工型的基因，并测定了其核苷酸序列。完整基因的长度为2134 bp。它由11个外显子和10个内含子组成。对糙皮侧耳072漆酶氨基酸序列的分析表明，它与糙皮侧耳属真菌分泌的其他漆酶具有高度同源性（高达96.9%）。
