Cooney Michael, Maynard Nathan, Cannizzaro Christopher, Benemann John
Hawaii Natural Energy Institute, University of Hawaii, 1680 East-West Road POST 109, Honolulu, HI 96822, USA.
Bioresour Technol. 2007 Oct;98(14):2641-51. doi: 10.1016/j.biortech.2006.09.054. Epub 2006 Dec 13.
An anaerobic digestion process to produce hydrogen and methane in two sequential stages was investigated, using two bioreactors of 2 and 15 L working volume, respectively. This relative volume ratio (and shorter retention time in the second, CH(4)-producing reactor) was selected, in part, to test the assumption that separation of phase can enhance metabolism in the second methane producing reactor. The reactor system was seeded with conventional anaerobic digester sludge, fed with a glucose-yeast extract--peptone medium and operated under conditions of relatively low mixing, to simulate full scale operation. A total of nine steady states were investigated, spanning a range of feed concentrations, dilution rates, feed carbon to nitrogen ratios and degree of integration of the two stages. The performance of this two-stage process and potential practical applications for the production of clean-burning hydrogen-methane mixtures are discussed.
研究了一种在两个连续阶段生产氢气和甲烷的厌氧消化过程，分别使用了工作体积为2升和15升的两个生物反应器。选择这种相对体积比（以及在第二个产甲烷反应器中较短的停留时间），部分原因是为了测试相分离可以增强第二个产甲烷反应器中新陈代谢的假设。该反应器系统接种了传统厌氧消化池污泥，以葡萄糖-酵母提取物-蛋白胨培养基为进料，并在相对低混合的条件下运行，以模拟实际规模运行。总共研究了九个稳态，涵盖了一系列进料浓度、稀释率、进料碳氮比以及两个阶段的整合程度。讨论了这种两阶段工艺的性能以及生产清洁燃烧氢气-甲烷混合物的潜在实际应用。
