Knezević-Vukcević J, Simić D
Laboratory for Microbiology, Faculty of Science, University of Belgrade, Yugoslavia.
Biochimie. 1991 Apr;73(4):497-500. doi: 10.1016/0300-9084(91)90118-k.
The repair response of Escherichia coli K-12 to bleomycin was examined in Rec- mutants showing differential sensitivity to this agent. Sedimentation analysis of the cellular DNA showed incision after bleomycin treatment. The subsequent reformation of the DNA, found in the wild-type and the recD mutant, was abolished in the recB and delayed in the recF and recBC sbcB mutants. The bleomycin-induced SOS response was reduced in strains containing recB or recBC sbsB mutations. It is suggested that the RecBCD pathway has the main role in the efficient repair of bleomycin-induced DNA damage.
在对博来霉素具有不同敏感性的Rec-突变体中，研究了大肠杆菌K-12对博来霉素的修复反应。对细胞DNA进行沉降分析显示，博来霉素处理后出现切口。野生型和recD突变体中随后出现的DNA重新形成，在recB突变体中被消除，在recF和recBC sbcB突变体中延迟。含有recB或recBC sbsB突变的菌株中，博来霉素诱导的SOS反应降低。提示RecBCD途径在博来霉素诱导的DNA损伤的有效修复中起主要作用。
