Carmichael A, Bateman N, Nayagam M
Department of Medicine, United Medical School, London.
Cytopathology. 1991;2(2):61-6. doi: 10.1111/j.1365-2303.1991.tb00388.x.
The results of the examination of sputum induced by the inhalation of nebulized hypertonic saline in the diagnosis of Pneumocystis carinii pneumonia (PCP) are presented. In suspected cases of PCP in patients who were either HIV antibody positive or were receiving immunosuppressive therapy, 46 induced sputum specimens were stained using both Grocott's modified Gomori methenamine silver nitrate (GMS) and immunofluorescence staining. In 12 specimens P. carinii cysts were detected by both methods, in four specimens by GMS staining only and in five specimens by immunofluorescence only. The sensitivity of induced sputum examination in the detection of P. carinii cysts was increased by using both of these staining methods on each sputum specimen and the need for more invasive methods of diagnosis was reduced.
本文展示了雾化吸入高渗盐水诱导痰液检查在卡氏肺孢子虫肺炎（PCP）诊断中的结果。在怀疑患有PCP的HIV抗体阳性患者或接受免疫抑制治疗的患者中，对46份诱导痰液标本同时使用改良格罗科特嗜银染色法（GMS）和免疫荧光染色法进行染色。在12份标本中，两种方法均检测到卡氏肺孢子虫包囊；4份标本仅通过GMS染色检测到；5份标本仅通过免疫荧光检测到。对每份痰液标本同时使用这两种染色方法，提高了诱导痰液检查在检测卡氏肺孢子虫包囊方面的敏感性，减少了对更具侵入性诊断方法的需求。
