评估单克隆抗体3F6在支气管肺泡灌洗标本和诱导痰中检测卡氏肺孢子虫肺炎的敏感性、特异性和预测价值。

Evaluation of the sensitivity, specificity, and predictive value of monoclonal antibody 3F6 for the detection of Pneumocystis carinii pneumonia in bronchoalveolar lavage specimens and induced sputum.

作者信息

Wazir J F, Macrorie S G, Coleman D V

机构信息

Department of Cytopathology, Saint Mary's Hospital, London, UK.

出版信息

Cytopathology. 1994 Apr;5(2):82-9. doi: 10.1111/j.1365-2303.1994.tb00531.x.

DOI:10.1111/j.1365-2303.1994.tb00531.x

PMID:7518707

Abstract

The sensitivity and specificity of different staining procedures for the detection of Pneumocystis carinii organisms were compared. Three conventional stains (Papanicolaou, Giemsa and Grocott's) and one immunocytochemical stain using 3F6 antibody were used on smears prepared from the same specimen. Bronchoalveolar lavage (BAL) and induced sputum (IS) specimens were used for this purpose. One hundred and sixty-five episodes from 142 patients were investigated by the four different staining techniques. Cysts of P. carinii were detected in 64 episodes from 63 patients. Immunocytochemical staining with 3F6 was found to be slightly more sensitive at detecting the cysts than Grocott's, Giemsa, or Papanicolaou stain.

摘要

比较了不同染色方法检测卡氏肺孢子虫生物体的敏感性和特异性。对取自同一标本制备的涂片使用了三种传统染色法（巴氏染色法、吉姆萨染色法和格罗科特染色法）以及一种使用3F6抗体的免疫细胞化学染色法。支气管肺泡灌洗（BAL）和诱导痰（IS）标本用于此目的。通过四种不同染色技术对142例患者的165次发病情况进行了调查。在63例患者的64次发病情况中检测到了卡氏肺孢子虫囊肿。发现用3F6进行免疫细胞化学染色在检测囊肿方面比格罗科特染色法、吉姆萨染色法或巴氏染色法略敏感。