Bahr B A, Sheppard A, Vanderklish P W, Bakus B L, Capaldi D, Lynch G
Center for the Neurobiology of Learning and Memory, University of California, Irvine 92717.
Neuroreport. 1991 Jun;2(6):321-4. doi: 10.1097/00001756-199106000-00005.
Immunochemical methods were used to test whether vitronectin receptors exist in synaptosomal membranes (SPMs) and hence are positioned to play a role in synaptic adhesion. Antibodies against the alpha v beta 3 integrin detected proteins in brain homogenates that correspond to conventional integrin subunits. Conversely, these antigens were not found in SPMs prepared from the same brain tissue. The antibodies did, however, express strong immunoreactivity towards a 27 kDa polypeptide that was greatly concentrated in SPMs from major brain regions and that was not found in tissues other than brain. This is an example of an integrin epitope contained in a synaptic polypeptide that is too small to be a conventional matrix receptor, thus, suggesting the possibility that synaptic adhesion involves unusual proteins.
采用免疫化学方法检测玻连蛋白受体是否存在于突触体膜（SPM）中，进而确定其是否在突触黏附中发挥作用。针对αvβ3整合素的抗体在脑匀浆中检测到与传统整合素亚基相对应的蛋白质。相反，在由相同脑组织制备的SPM中未发现这些抗原。然而，这些抗体确实对一种27 kDa的多肽表现出强烈的免疫反应性，该多肽在大脑主要区域的SPM中高度富集，在脑组织以外的其他组织中未发现。这是一个存在于突触多肽中的整合素表位的例子，该表位太小而无法成为传统的基质受体，因此，提示突触黏附可能涉及非寻常蛋白质的可能性。
