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从脑突触质膜中纯化精氨酸-甘氨酸-天冬氨酸选择性基质受体。

Purification of an Arg-Gly-Asp selective matrix receptor from brain synaptic plasma membranes.

作者信息

Bahr B A, Lynch G

机构信息

Center for the Neurobiology of Learning and Memory, University of California, Irvine 92717-3800.

出版信息

Biochem J. 1992 Jan 1;281 ( Pt 1)(Pt 1):137-42. doi: 10.1042/bj2810137.

DOI:10.1042/bj2810137
PMID:1731748
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1130651/
Abstract

Brain synaptic plasma membranes specifically associated with matrix protein monolayers containing the Arg-Gly-Asp sequence recognized by integrin-type adhesion receptors. Experiments using fibronectin affinity chromatography to identify the synaptosomal receptors responsible for this interaction led to the purification of a 55 kDa Arg-Gly-Asp recognition protein that is labelled by antibodies against the alpha 5 beta 1 integrin.

摘要

脑突触质膜与含有被整合素型黏附受体识别的精氨酸-甘氨酸-天冬氨酸序列的基质蛋白单层特异性相关。使用纤连蛋白亲和层析来鉴定负责这种相互作用的突触体受体的实验，导致纯化出一种55 kDa的精氨酸-甘氨酸-天冬氨酸识别蛋白，该蛋白被抗α5β1整合素的抗体标记。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/401b/1130651/b08444f93652/biochemj00144-0143-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/401b/1130651/bd783737ab92/biochemj00144-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/401b/1130651/e627e6751380/biochemj00144-0143-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/401b/1130651/b08444f93652/biochemj00144-0143-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/401b/1130651/bd783737ab92/biochemj00144-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/401b/1130651/e627e6751380/biochemj00144-0143-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/401b/1130651/b08444f93652/biochemj00144-0143-b.jpg

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Isolation and characterization of a collagen-binding glycoprotein from chondrocyte membranes.从软骨细胞膜中分离并鉴定一种胶原结合糖蛋白。
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Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule.
整合素亚基基因表达在成体大脑中呈区域分化。
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The relationship between adhesion molecules and neuronal plasticity.黏附分子与神经元可塑性之间的关系。
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Arg-Gly-Asp-Ser-selective adhesion and the stabilization of long-term potentiation: pharmacological studies and the characterization of a candidate matrix receptor.精氨酸-甘氨酸-天冬氨酸-丝氨酸选择性黏附与长时程增强的稳定：药理学研究及一种候选基质受体的特性分析
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Characterization of densin-180, a new brain-specific synaptic protein of the O-sialoglycoprotein family.致密素-180的特性研究，一种新的O-唾液酸糖蛋白家族脑特异性突触蛋白。
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纤连蛋白的细胞附着活性可以被该分子的小合成片段复制。
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Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.聚丙烯酰胺凝胶中蛋白质的超敏染色显示脑脊液蛋白质存在区域差异。
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