Migliaccio G, Migliaccio A R, Druzin M L, Giardina P J, Zsebo K M, Adamson J W
Laboratory of Hematopoietic Growth Factors, New York Blood Center, New York 10021.
J Cell Physiol. 1991 Sep;148(3):503-9. doi: 10.1002/jcp.1041480324.
We have studied the effect of recombinant human Stem Cell Factor (SCF) on the growth of human peripheral blood, bone marrow, and cord blood progenitor cells in semisolid medium. While SCF alone had little colony-stimulating activity under fetal bovine serum (FBS)-deprived culture conditions, SCF synergized with erythropoietin (Epo), granulocyte/macrophage colony-stimulating factor (GM-CSF), and interleukin 3 (IL-3) to stimulate colony growth. Colony morphology was determined by the late-acting growth factor added along with SCF. Of all the combinations of growth factors, SCF plus IL-3 and Epo resulted in the largest number of mixed-cell colonies--a larger number than observed with IL-3 and Epo alone even in FBS-supplemented cultures. These results suggest that SCF is a growth factor that more specifically targets early progenitor cells (mixed-cell colony-forming cells) and has the capacity to synergize with a wide variety of other hematopoietic growth factors to cause the proliferation and differentiation of committed progenitor cells. Our studies indicate that SCF may be the earliest acting growth factor described to date.
我们研究了重组人干细胞因子（SCF）对人外周血、骨髓和脐血祖细胞在半固体培养基中生长的影响。在缺乏胎牛血清（FBS）的培养条件下，单独的SCF几乎没有集落刺激活性，但SCF可与促红细胞生成素（Epo）、粒细胞/巨噬细胞集落刺激因子（GM-CSF）和白细胞介素3（IL-3）协同作用以刺激集落生长。集落形态由与SCF一起添加的晚期作用生长因子决定。在所有生长因子组合中，SCF加IL-3和Epo产生的混合细胞集落数量最多——即使在补充FBS的培养物中，该数量也比单独使用IL-3和Epo时观察到的数量更多。这些结果表明，SCF是一种更特异性地作用于早期祖细胞（混合细胞集落形成细胞）的生长因子，并且有能力与多种其他造血生长因子协同作用，从而导致定向祖细胞的增殖和分化。我们的研究表明，SCF可能是迄今为止所描述的最早起作用的生长因子。
