Bai H W, Shi B Y, Qian Y Y, Na Y Q, Cai M, Zeng X, Zhong D R, Wu S F, Chang J Y, Zhou W Q
Organ Transplantation Center of PLA, Second Affiliated Hospital of PLA General Hospital, Beijing, China.
Transplant Proc. 2006 Dec;38(10):3430-3. doi: 10.1016/j.transproceed.2006.10.154.
The blood vessels of a transplanted organ are an interface between the donor and the recipient. The endothelium is believed to be a major target for graft rejection. After transplantation endothelial cells of a transplanted organ may be of recipient origin.
In this study we sought to determine whether endothelial chimerism correlates with graft rejection.
Biopsy samples from 34 renal transplants of female recipients who received kidneys from male donors were studied for the presence of endothelial cells of recipient origin. Formalin-fixed, paraffin-embedded tissue sections of renal biopsy samples were examined by fluorescence in situ hybridization (FISH) for the presence of endothelial cells containing two X chromosomes, using a biotinylated Y-chromosome probe and digoxigenin-labeled X-chromosome probe.
The FISH methods identified endothelial cells of recipient origin. Endothelial chimerism was common, irrespective of rejection. Its presence was focal with these elements, coexisting in the biopsy.
We observed no correlation between the percentage of recipient endothelial cells among vascular elements and the type of graft rejection (P > .05).
移植器官的血管是供体与受体之间的一个界面。内皮细胞被认为是移植排斥反应的主要靶点。移植后，移植器官的内皮细胞可能来源于受体。
在本研究中，我们试图确定内皮嵌合现象是否与移植排斥反应相关。
对34例接受男性供体肾脏的女性肾移植受者的活检样本进行研究，以检测受体来源的内皮细胞。使用生物素化的Y染色体探针和地高辛标记的X染色体探针，通过荧光原位杂交（FISH）对肾活检样本的福尔马林固定、石蜡包埋组织切片进行检查，以检测含有两条X染色体的内皮细胞。
FISH方法鉴定出了受体来源的内皮细胞。内皮嵌合现象很常见，与排斥反应无关。其存在是局灶性的，这些成分在活检中并存。
我们观察到血管成分中受体内皮细胞的百分比与移植排斥反应类型之间无相关性（P>.05）。
