Bai Hong-wei, Shi Bing-yi, Qian Ye-yong, Na Yan-qun, Zeng Xuan, Zhong Ding-rong, Lu Min, Zou Wan-zhong, Wu Sha-fei
Transplantation Centre of PLA, Second Affiliated Hospital of PLA General Hospital, Beijing 100091, China.
Chin Med J (Engl). 2007 May 20;120(10):859-62.
The blood vessels of a transplanted organ are the interface between donor and recipient. The endothelium in the blood vessels is thought to be the major target for graft rejection. Endothelial cells of a transplanted organ can be of recipient origin after transplantation. In this study, we tested whether endothelial chimerism correlated with the graft rejection and cold ischemia.
We studied the biopsy samples from 34 renal transplants of female recipients who received the kidney from a male donor for the presence of endothelial cells of recipient origin. We examined the tissue sections of renal biopsy samples by fluorescence in situ hybridization (FISH) for the presence of endothelial cells containing two X chromosomes using a biotinylated Y chromosome probe and digoxigenin labelled X chromosome probe, and then analyzed the relationship between the endothelial cell chimerism and the rejection and cold ischemia.
Endothelial chimerism was common and irrespective of rejections (P > 0.05). The cold ischemic time of chimerism group was longer than no chimerism group ((14.83 +/- 4.03) hours vs (11.27 +/- 3.87) hours, P < 0.05).
There is no correlation between the percentage of recipient endothelial cells in vascular endothelial cells and the type of graft rejection. The endothelium damaged by ischemic injury might be repaired by the endothelial cells from the recipient.
移植器官的血管是供体和受体之间的界面。血管内皮被认为是移植排斥的主要靶点。移植器官的内皮细胞在移植后可能来源于受体。在本研究中，我们测试了内皮嵌合现象是否与移植排斥及冷缺血相关。
我们研究了34例女性肾移植受者的活检样本，这些受者接受了来自男性供体的肾脏，以检测受体来源的内皮细胞。我们使用生物素化的Y染色体探针和地高辛标记的X染色体探针，通过荧光原位杂交（FISH）检查肾活检样本的组织切片，以检测含有两条X染色体的内皮细胞，然后分析内皮细胞嵌合现象与排斥反应及冷缺血之间的关系。
内皮嵌合现象很常见，且与排斥反应无关（P>0.05）。嵌合组的冷缺血时间长于非嵌合组（（14.83±4.03）小时对（11.27±3.87）小时，P<0.05）。
血管内皮细胞中受体来源内皮细胞的百分比与移植排斥类型之间无相关性。缺血性损伤所损伤的内皮可能由受体的内皮细胞修复。
