合成双链RNA（聚肌苷酸：聚胞苷酸，poly rI:rC）在体内对干扰素诱导基因的激活作用。

Activation of interferon-inducible genes in vivo by synthetic double-stranded RNA, poly rI:rC.

作者信息

Gariglio M, Panico S, Gribaudo G, Martinotti M G, Cavallo G, Landolfo S

机构信息

Institute of Microbiology, Medical School, University of Turin, Italy.

出版信息

Microbiologica. 1991 Jul;14(3):179-83.

PMID:1717810

Abstract

We analyzed the effects of synthetic dsRNA (poly rI:rC) treatment on the expression in vivo of two interferon (IFN)-inducible genes. DBA/2 mice were injected i.p. with poly rI:rC and its effect on the levels of the following mRNAs was determined; 202, 2'-5' oligoadenylate synthetase (2-5A synthetase) and beta-actin. After poly rI:rC treatment the levels of the 202 and 2-5A synthetase mRNAs in the spleen and in bone marrow peaked between 12 and 24 h and decreased thereafter, whereas beta-actin levels remained unchained unchanged. Pretreatment of DBA/2 mice with sheep anti-murine IFN-alpha/beta antibodies before rI:rC injection strongly diminished the induction of 202 mRNA indicating that IFN-alpha/beta mediated this induction.

摘要

我们分析了合成双链RNA（聚肌苷酸：聚胞苷酸，poly rI:rC）处理对两种干扰素（IFN）诱导基因体内表达的影响。给DBA/2小鼠腹腔注射poly rI:rC，并测定其对以下mRNA水平的影响：202、2'-5'寡腺苷酸合成酶（2-5A合成酶）和β-肌动蛋白。poly rI:rC处理后，脾脏和骨髓中202和2-5A合成酶mRNA的水平在12至24小时之间达到峰值，随后下降，而β-肌动蛋白水平保持不变。在注射rI:rC之前，用羊抗鼠IFN-α/β抗体对DBA/2小鼠进行预处理，可强烈减弱202 mRNA的诱导，表明IFN-α/β介导了这种诱导。