Stergiopoulos Ioannis, Groenewald Marizeth, Staats Martijn, Lindhout Pim, Crous Pedro W, De Wit Pierre J G M
Laboratory of Phytopathology, Wageningen University and Research Centre, Binnenhaven 5, 6709 PD Wageningen, The Netherlands.
Fungal Genet Biol. 2007 May;44(5):415-29. doi: 10.1016/j.fgb.2006.11.004. Epub 2006 Dec 18.
Two mating-type genes, designated MAT1-1-1 and MAT1-2-1, were cloned and sequenced from the presumed asexual ascomycete Cladosporium fulvum (syn. Passalora fulva). The encoded products are highly homologous to mating-type proteins from members of the Mycosphaerellaceae, such as Mycosphaerella graminicola and Cercospora beticola. In addition, the two MAT idiomorphs of C. fulvum showed regions of homology and each contained one additional putative ORF without significant similarity to known sequences. The distribution of the two mating-type genes in a world-wide collection of 86 C. fulvum strains showed a departure from a 1:1 ratio (chi(2)=4.81, df=1). AFLP analysis revealed a high level of genotypic diversity, while strains of the fungus were identified with similar virulence spectra but distinct AFLP patterns and opposite mating-types. These features could suggest the occurrence of recombination in C. fulvum.
从假定的无性子囊菌番茄叶霉（Cladosporium fulvum，同物异名：Passalora fulva）中克隆并测序了两个交配型基因，命名为MAT1-1-1和MAT1-2-1。编码产物与球腔菌科成员（如小麦壳针孢和甜菜尾孢）的交配型蛋白高度同源。此外，番茄叶霉的两个MAT特异性基因座显示出同源区域，并且每个都包含一个与已知序列无明显相似性的额外推定开放阅读框。在全球收集的86株番茄叶霉菌株中，这两个交配型基因的分布偏离了1:1的比例（χ2 = 4.81，自由度 = 1）。AFLP分析揭示了高水平的基因型多样性，而该真菌的菌株具有相似的毒力谱，但AFLP模式不同且交配型相反。这些特征可能表明番茄叶霉中发生了重组。
