Curtis M D, Gore J, Oliver R P
Norwich Molecular Plant Pathology Group, School of Biological Sciences, University of East Anglia, UK.
Curr Genet. 1994 Apr;25(4):318-22. doi: 10.1007/BF00351484.
The nucleotide sequence of part of the ribosomal DNA from races of the fungal tomato pathogen Cladosporium fulvum and other Cladosporium species have been determined. Comparisons of the internal transcribed spacer regions (ITS1 and ITS2) of several C. fulvum races showed complete sequence homology suggesting a recent evolutionary divergence. Comparisons of these nucleotide sequences in the ITS region with those of other Cladosporium species showed the close relationship within the Cladosporium genus. Using the nucleotide sequence of part of the 18s ribosomal subunit from these isolates and comparing them with sequences of some Ascomycetes, Basidiomycetes and Chytridiomycetes, obtained from GenBank, we infer the phylogeny of the Cladosporium species studied here. Our analysis shows that the Cladosporia form a monophyletic group which falls within the order Ascomycotina.
已测定了番茄真菌病原体fulvum枝孢菌不同小种以及其他枝孢菌物种核糖体DNA部分的核苷酸序列。对fulvum枝孢菌几个小种的内部转录间隔区（ITS1和ITS2）进行比较，结果显示序列完全同源，表明其进化分歧时间较近。将这些ITS区域的核苷酸序列与其他枝孢菌物种的序列进行比较，结果显示枝孢菌属内关系密切。利用这些分离株18s核糖体亚基部分的核苷酸序列，并将其与从GenBank获得的一些子囊菌、担子菌和壶菌的序列进行比较，我们推断了此处所研究枝孢菌物种的系统发育。我们的分析表明，枝孢菌形成了一个单系类群，属于子囊菌纲。
