Jakubs Katherine, Nanobashvili Avtandil, Bonde Sara, Ekdahl Christine T, Kokaia Zaal, Kokaia Merab, Lindvall Olle
Laboratory of Neurogenesis and Cell Therapy, Section of Restorative Neurology, Wallenberg Neuroscience Center, University Hospital, SE-221 84 Lund, Sweden.
Neuron. 2006 Dec 21;52(6):1047-59. doi: 10.1016/j.neuron.2006.11.004.
Neural progenitors in the adult dentate gyrus continuously produce new functional granule cells. Here we used whole-cell patch-clamp recordings to explore whether a pathological environment influences synaptic properties of new granule cells labeled with a GFP-retroviral vector. Rats were exposed to a physiological stimulus, i.e., running, or a brain insult, i.e., status epilepticus, which gave rise to neuronal death, inflammation, and chronic seizures. Granule cells formed after these stimuli exhibited similar intrinsic membrane properties. However, the new neurons born into the pathological environment differed with respect to synaptic drive and short-term plasticity of both excitatory and inhibitory afferents. The new granule cells formed in the epileptic brain exhibited functional connectivity consistent with reduced excitability. We demonstrate a high degree of plasticity in synaptic inputs to adult-born new neurons, which could act to mitigate pathological brain function.
成年齿状回中的神经祖细胞持续产生新的功能性颗粒细胞。在此，我们使用全细胞膜片钳记录来探究病理环境是否会影响用绿色荧光蛋白逆转录病毒载体标记的新颗粒细胞的突触特性。将大鼠暴露于生理刺激（即跑步）或脑部损伤（即癫痫持续状态，这会导致神经元死亡、炎症和慢性癫痫发作）。这些刺激后形成的颗粒细胞表现出相似的内在膜特性。然而，在病理环境中产生的新神经元在兴奋性和抑制性传入神经的突触驱动和短期可塑性方面存在差异。癫痫脑中形成的新颗粒细胞表现出与兴奋性降低相一致的功能连接。我们证明成年新生神经元的突触输入具有高度可塑性，这可能有助于减轻病理性脑功能。
