Carretero J, Sánchez F, Vázquez R, Cacicedo L, Sánchez-Franco F, Fernández G, Montero M
Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, Spain.
Neuropeptides. 1991 Aug;19(4):223-9. doi: 10.1016/0143-4179(91)90088-z.
In order to characterize immunocytochemically the existence of GRF in the rat adenohypophysis and the origin of this hormone, an immunocytochemical and morphometric study was made of r-GRF-immunoreactive cells from the adenohypophysis of untreated adult rats, adult rats treated intraventricularly with colchicine and in primary cultures of adult rat adenohypophyseal cells that had been incubated with serum devoid of GRF. r-GRF immunoreactive cells were observed in untreated rat pituitaries, both male and female, although the numbers of positive cells were greater in the males (p less than 0.05) and were found to increase in number following treatment with colchicine (p less than 0.01). These cells appeared dispersed throughout the anterior lobe, without forming clusters, and were often close to blood vessels. Additionally, immunoreactive cells appeared in the cultures at 7 days of incubation. The presence of GRF-immunoreactive cells in the adenohypophysis of rats previously treated with colchicine suggests the existence of a non-hypothalamic origin for r-GRF; this is confirmed by the findings obtained in the in vitro studies which would corroborate the hypothesis that the origin of the neuropeptide is in the rat adenohypophysis itself.
为了通过免疫细胞化学方法鉴定大鼠腺垂体中生长激素释放因子（GRF）的存在及其激素来源，对未处理的成年大鼠、经脑室注射秋水仙碱处理的成年大鼠以及在不含GRF的血清中孵育的成年大鼠腺垂体细胞原代培养物中的r-GRF免疫反应性细胞进行了免疫细胞化学和形态计量学研究。在未处理的大鼠垂体中观察到r-GRF免疫反应性细胞，无论雌雄，尽管雄性中的阳性细胞数量更多（p<0.05），并且发现用秋水仙碱处理后细胞数量增加（p<0.01）。这些细胞似乎分散在前叶各处，不形成簇，且常靠近血管。此外，在培养7天时出现了免疫反应性细胞。先前用秋水仙碱处理的大鼠腺垂体中存在GRF免疫反应性细胞，这表明r-GRF存在非下丘脑来源；体外研究的结果证实了这一点，这些结果支持了神经肽起源于大鼠腺垂体本身的假说。
