Carretero J, Sánchez F, Rubio M, Juanes J A, Blanco E, González R, Riesco J M, Vázquez R
Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, Spain.
Histol Histopathol. 1992 Oct;7(4):673-7.
In order to elucidate whether the gender differences observed in the somatotropic cells of adult rats are mediated by hypothalamic neuropeptides, a morphometric analysis was made of the GH-immunoreactive cells of adult rats treated intraventricularly with colchicine. The morphometric and morphological findings obtained were correlated to the basal serum levels of GH at the time of sacrifice. Treatment with colchicine was seen to increase serum GH levels; this increase was accompanied by an increase in the intensity of the reaction of the GH-cells and, morphometrically, an increase in their size due to an increase in the nuclear area, but with no significant changes in the cytoplasmic area. The results suggest that in the absence of somatostatin and GRF the basal release of GH is elevated in a similar fashion in both sexes, in turn suggesting that gonadal steroids might act at hypothalamic level on the release of somatostatin and, indirectly, on the intracellular pool of GH and hormonal secretion.
为了阐明成年大鼠生长激素细胞中观察到的性别差异是否由下丘脑神经肽介导，对脑室注射秋水仙碱的成年大鼠的生长激素免疫反应性细胞进行了形态计量分析。所获得的形态计量学和形态学结果与处死时生长激素的基础血清水平相关。秋水仙碱治疗可使血清生长激素水平升高；这种升高伴随着生长激素细胞反应强度的增加，从形态计量学来看，由于核面积增加，细胞大小也增加，但细胞质面积无显著变化。结果表明，在缺乏生长抑素和生长激素释放因子的情况下，两性生长激素的基础释放以类似方式升高，这反过来表明性腺类固醇可能在下丘脑水平作用于生长抑素的释放，并间接作用于生长激素的细胞内池和激素分泌。
