Queloz Pierre-Alain, Crettaz David, Thadikkaran Lynne, Sapin Vincent, Gallot Denis, Jani Jacques, Deprest Jan, Lémery Didier, Barelli Stefano, Tissot Jean-Daniel
Service Régional Vaudois de Transfusion Sanguine, Lausanne, Switzerland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 May 1;850(1-2):336-42. doi: 10.1016/j.jchromb.2006.12.006. Epub 2006 Dec 19.
Amniotic fluid (AF) is a potential source of biomarkers for many disorders which may occur during pregnancy. The purpose of this study was to evaluate the place of two-dimensional gel electrophoresis (2-DE) technologies to compare AF in both normal and pathological situations. Two-dimensional fluorescence difference gel electrophoresis (2D-DIGE; Ettan DIGE) as well as two-dimensional gel electrophoresis and silver staining followed by image analysis were used. Differentially expressed proteins were identified by mass spectrometry. This approach was used to study electrophoregrams of normal AF obtained at 17 weeks of gestation and at term, as well as AF from fetuses presenting with congenital diaphragmatic hernia. Finally, the potential of two-dimensional electrophoresis was assessed by studying the protein profile of plasma containing AF proteins in a model of premature rupture of the membranes (PROM). Our results clearly show that two-dimensional electrophoresis technologies still have place for analyzing biological fluids such as AF.
羊水（AF）是许多孕期可能出现的疾病生物标志物的潜在来源。本研究的目的是评估二维凝胶电泳（2-DE）技术在比较正常和病理情况下羊水方面的作用。采用了二维荧光差异凝胶电泳（2D-DIGE；Ettan DIGE）以及二维凝胶电泳和银染后进行图像分析的方法。通过质谱鉴定差异表达的蛋白质。该方法用于研究妊娠17周时和足月时获得的正常羊水的电泳图谱，以及患有先天性膈疝胎儿的羊水电泳图谱。最后，通过研究胎膜早破（PROM）模型中含有羊水蛋白的血浆蛋白质谱，评估二维电泳的潜力。我们的结果清楚地表明，二维电泳技术在分析羊水等生物体液方面仍有作用。
