Park Soo-Jin, Yoon Won-Gap, Song Jin-Su, Jung Hyun Sook, Kim Chong Jai, Oh Soo Young, Yoon Bo Hyun, Jung Guhung, Kim Hie-Joon, Nirasawa Takashi
Department of Chemistry, Seoul National University, Gwanak-gu, Seoul, Korea 151-747.
Proteomics. 2006 Jan;6(1):349-63. doi: 10.1002/pmic.200500084.
Proteome analysis by 2-DE and PMF by MALDI-TOF MS was performed on human amnion and amniotic fluid at term. Ninety-two soluble and nineteen membrane proteins were identified from amnion. Thirty-five proteins were identified from amniotic fluid. Calgranulin A and B were found in all patients infected with Ureaplasma urealyticum, but not in any of the patients without infection, indicating that they are potential markers of intrauterine infection. Identity of calgranulin A and B was confirmed by MALDI-TOF/TOF MS. This study represents the first extensive analysis of the human amnion and amniotic fluid proteome at term and demonstrates that 2-DE and MALDI-TOF MS is a useful tool for identifying clinically significant biomarkers of problematic pregnancies.
采用二维电泳（2-DE）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）对足月时的人羊膜和羊水进行蛋白质组分析。从羊膜中鉴定出92种可溶性蛋白和19种膜蛋白。从羊水中鉴定出35种蛋白。在所有解脲脲原体感染的患者中均发现了钙粒蛋白A和B，但在未感染的患者中均未发现，这表明它们是宫内感染的潜在标志物。通过MALDI-TOF/TOF MS确认了钙粒蛋白A和B的身份。本研究首次对足月时的人羊膜和羊水蛋白质组进行了广泛分析，并证明二维电泳和MALDI-TOF MS是鉴定妊娠问题临床显著生物标志物的有用工具。
