Daimon M, Wang C Y, Johnson T R, Ilan J, Ilan J
Department of Reproductive Biology, Case Western Reserve University School of Medicine, Cleveland, Ohio.
Mol Reprod Dev. 1991 Jul;29(3):238-44. doi: 10.1002/mrd.1080290305.
A cDNA clone of 525 bp corresponding to the 3'-untranslated region of insulin-like growth factor-I was isolated from a human placenta library. The sequence of this clone extended 200 nucleotides downstream from the previously reported 3'-end of IGF-IA cDNA, indicating the existence of IGF-IA transcripts having an even larger 3'-untranslated region. By using this clone for RNA transfer blot hybridization, it was shown that this longer 3'-untranslated region is included in the 7.5- and 5.0-kb transcripts, but not in the 1.1- and 0.9-kb transcripts. It is also apparent that transcripts bearing the extended 3'-untranslated sequence are highly expressed in human placenta.
从人胎盘文库中分离出一个与胰岛素样生长因子-I的3'-非翻译区相对应的525bp的cDNA克隆。该克隆的序列从先前报道的IGF-IA cDNA的3'-末端向下游延伸了200个核苷酸，表明存在具有更大3'-非翻译区的IGF-IA转录本。通过使用该克隆进行RNA转移印迹杂交，结果表明这个更长的3'-非翻译区包含在7.5kb和5.0kb的转录本中，但不包含在1.1kb和0.9kb的转录本中。同样明显的是，带有延伸的3'-非翻译序列的转录本在人胎盘中高度表达。
