Chassin D, Laurent A, Janneau J L, Berger R, Bellet D
Laboratoire d'Immunologie des Tumeurs, URA 1484 CNRS, Université René Descartes, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, France.
Genomics. 1995 Sep 20;29(2):465-70. doi: 10.1006/geno.1995.9980.
A new member of the insulin gene superfamily was identified by screening a subtracted cDNA library of first-trimester human placenta and, hence, was tentatively named early placenta insulin-like peptide (EPIL). In this paper, we report the cloning and sequencing of the EPIL cDNA and the EPIL gene (INSL4). Comparison of the deduced amino acid sequence of the early placenta insulin-like peptide revealed significant overall and structural homologies with members of the insulin-like hormone superfamily. Moreover, the organization of the early placenta insulin-like gene, which is composed of two exons and one intron, is similar to that of insulin and relaxin. By in situ hybridization, the INSL4 gene was assigned to band p24 of the short arm of chromosome 9. RT-PCR analysis of EPIL tissue distribution revealed that its transcripts are expressed in the placenta and uterus.
通过筛选孕早期人胎盘的消减cDNA文库，鉴定出胰岛素基因超家族的一个新成员，因此暂将其命名为早期胎盘胰岛素样肽（EPIL）。在本文中，我们报告了EPIL cDNA和EPIL基因（INSL4）的克隆与测序。早期胎盘胰岛素样肽推导的氨基酸序列与胰岛素样激素超家族成员相比，在整体和结构上具有显著同源性。此外，早期胎盘胰岛素样基因由两个外显子和一个内含子组成，其结构与胰岛素和松弛素的基因结构相似。通过原位杂交，将INSL4基因定位于9号染色体短臂的p24带。对EPIL组织分布的RT-PCR分析表明，其转录本在胎盘和子宫中表达。
