Kyan-Aung U, Haskard D O, Lee T H
Department of Allergy, United Medical School, Guy's Hospital, London, United Kingdom.
Am J Respir Cell Mol Biol. 1991 Nov;5(5):445-50. doi: 10.1165/ajrcmb/5.5.445.
Cultured human umbilical vein endothelial cell (EC) monolayers stimulated with 10 ng/ml tumor necrosis factor demonstrate a time-dependent increase in the expression of the vascular cell adhesion molecule-1 (VCAM-1) with maintained maximal expression at 24 h following EC activation. A monoclonal antibody (mAb) directed against VCAM-1 (1G11) significantly inhibited the adhesion of eosinophils, but not neutrophils, to EC, which had been activated by tumor necrosis factor-alpha for 24 h, but only when eosinophils had been pretreated with an mAb directed against the common beta chain of the CD11/CD18 complex. In the absence of pretreatment with anti-CD18, mAb 1G11 had no significant effect on eosinophil adhesion. These results suggest that eosinophils bind to VCAM-1. However, the functional capacity in this model of the eosinophil receptor for VCAM-1 is likely to be minor compared with the activity of the CD11/CD18 leukocyte adhesion molecules.
用10纳克/毫升肿瘤坏死因子刺激培养的人脐静脉内皮细胞（EC）单层，血管细胞黏附分子-1（VCAM-1）的表达呈时间依赖性增加，在EC激活后24小时保持最大表达。一种针对VCAM-1的单克隆抗体（mAb，1G11）显著抑制嗜酸性粒细胞而非中性粒细胞与已被肿瘤坏死因子-α激活24小时的EC的黏附，但仅当嗜酸性粒细胞用针对CD11/CD18复合物共同β链的mAb预处理时才有效。在没有抗CD18预处理的情况下，mAb 1G11对嗜酸性粒细胞黏附没有显著影响。这些结果表明嗜酸性粒细胞与VCAM-1结合。然而，在该模型中，嗜酸性粒细胞VCAM-1受体的功能能力与CD11/CD18白细胞黏附分子的活性相比可能较小。
