Gavin Amanda L, Hoebe Kasper, Duong Bao, Ota Takayuki, Martin Christopher, Beutler Bruce, Nemazee David
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037 USA.
Science. 2006 Dec 22;314(5807):1936-8. doi: 10.1126/science.1135299.
Innate immune signals mediated by Toll-like receptors (TLRs) have been thought to contribute considerably to the antibody-enhancing effects of vaccine adjuvants. However, we report here that mice deficient in the critical signaling components for TLR mount robust antibody responses to T cell-dependent antigen given in four typical adjuvants: alum, Freund's complete adjuvant, Freund's incomplete adjuvant, and monophosphoryl-lipid A/trehalose dicorynomycolate adjuvant. We conclude that TLR signaling does not account for the action of classical adjuvants and does not fully explain the action of a strong adjuvant containing a TLR ligand. This may have important implications in the use and development of vaccine adjuvants.
由Toll样受体(TLR)介导的先天免疫信号一直被认为在很大程度上促成了疫苗佐剂的抗体增强效应。然而,我们在此报告,缺乏TLR关键信号成分的小鼠对四种典型佐剂(明矾、弗氏完全佐剂、弗氏不完全佐剂和单磷酰脂质A/海藻糖二分枝菌酸佐剂)中给予的T细胞依赖性抗原产生了强烈的抗体反应。我们得出结论,TLR信号传导并不能解释传统佐剂的作用,也不能完全解释含有TLR配体的强效佐剂的作用。这可能对疫苗佐剂的使用和开发具有重要意义。