Friedman David B
Department of Biochemistry, Mass Spectrometry Research Center, Nashville, TN, USA.
Methods Mol Biol. 2007;367:219-39. doi: 10.1385/1-59745-275-0:219.
Difference gel electrophoresis (DIGE) technology provides a powerful quantitative component to proteomics experiments involving two-dimensional (2D) gel electrophoresis. DIGE allows for the detection of subtle changes in protein abundance with statistical confidence while controlling for gel-to-gel variation, as well as additional variation that is non-biological in origin (e.g., sample preparation error, normal variation in a system). Samples are differentially labeled with spectrally resolvable fluorescent dyes (Cy2, Cy3, and Cy5) and co-resolved for direct quantification within the same 2D gel. Increased statistical confidence is obtained when combining experimental repetition with internal standards such that independent replicate measurements from single- and multivariable analyses can be intercompared with a relatively small number of coordinated DIGE gels.
差异凝胶电泳（DIGE）技术为涉及二维（2D）凝胶电泳的蛋白质组学实验提供了强大的定量分析手段。DIGE能够在控制凝胶间差异以及其他非生物学来源的差异（例如样品制备误差、系统中的正常变异）的同时，以统计学置信度检测蛋白质丰度的细微变化。样品用可光谱分辨的荧光染料（Cy2、Cy3和Cy5）进行差异标记，并在同一2D凝胶中共同分离以进行直接定量。当将实验重复与内标相结合时，可以获得更高的统计学置信度，这样来自单变量和多变量分析的独立重复测量结果就可以在相对较少数量的协同DIGE凝胶之间进行相互比较。
