Majtán Viktor, Majtán Tomás, Majtán Juraj, Szabóová Monika, Majtánová L'ubica
Department of Microbiology, Slovak Medical Univeristy, Bratislava, Slovak Republic.
Jpn J Infect Dis. 2006 Dec;59(6):358-62.
A collection of 68 isolates of Salmonella enterica serovar Kentucky collected during the period 2003-2004 from humans in two geographical regions in the Slovak Republic was studied. The original isolate of this serovar was associated with travel to Egypt, and the emergence of other isolates was due to the nosocomial spread of this strain in two hospitals. Antimicrobial susceptibility testing, class I integrons content, pulsed-field gel electrophoresis (PFGE) analysis and plasmid DNA profiles were performed on all isolates. A high proportion (89.7%) of the isolates was multidrug-resistant, while 67 strains expressed resistance against ciprofloxacin. By sequence analysis of randomly selected strains, the point mutations in quinolone resistance-determining region of the DNA gyrase were found. The S. Kentucky isolates investigated were determined to be clonally related by PFGE as well as plasmid DNA analysis.
对2003年至2004年期间从斯洛伐克共和国两个地理区域的人类中收集的68株肠炎沙门氏菌肯塔基血清型分离株进行了研究。该血清型的原始分离株与前往埃及旅行有关，其他分离株的出现是由于该菌株在两家医院的医院内传播。对所有分离株进行了抗菌药敏试验、I类整合子含量分析、脉冲场凝胶电泳（PFGE）分析和质粒DNA图谱分析。高比例（89.7%）的分离株对多种药物耐药，而67株对环丙沙星耐药。通过对随机选择的菌株进行序列分析，发现了DNA回旋酶喹诺酮耐药决定区的点突变。通过PFGE以及质粒DNA分析确定，所研究的肯塔基沙门氏菌分离株具有克隆相关性。
