Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Copenhagen, Denmark.
PLoS One. 2013;8(3):e57811. doi: 10.1371/journal.pone.0057811. Epub 2013 Mar 6.
Contaminated poultry and poultry products are a major source of motile Salmonellae for human salmonellosis worldwide. Local circulation of any motile Salmonella serovar in poultry has a wider public health impact beyond its source of origin for being dispersed elsewhere through poultry trades or human travels. To investigate the status of motile Salmonella serovars in breeder farms in Bangladesh, multiple flocks of two breeder farms were observed for a period of six months. In addition, a cross-sectional survey was carried out to determine the prevalence and serovar distribution of motile Salmonella by randomly selecting 100 commercial broiler poultry farms. Five pooled faecal samples representing an entire housed flock of breeders or broilers were screened for presence of motile Salmonella following conventional bacteriological procedures. The Salmonella isolates obtained were subsequently serotyped, and characterized by plasmid profiling and pulsed-field gel electrophoresis (PFGE). The results revealed that both the breeder farms were positive with three Salmonella serovars: S. Virchow, S. Paratyphi B var Java (S. Java) and S. Enteritidis. Eleven of the 100 broiler farms investigated were positive for motile Salmonella, giving a farm-level prevalence of 11% (95% confidence interval 5-17%). S. Virchow and S. Kentucky were the two predominant serovars isolated from the broiler farms. The PFGE genotyping demonstrated that the isolates belonging to the same serovars were closely related due to variation in only 1-4 bands. All the S. Virchow and S. Java isolates, irrespective of breeder or broiler farm origin, were plasmid-free, except for one S. Virchow isolate from a broiler farm that harboured a 9.7 kb-sized plasmid. The S. Kentucky isolates belonged to three plasmid profiles having plasmids of four different sizes, ranging from 2.7 to 109 kb. This is the first report of any motile Salmonella serovars from breeder and commercial broiler poultry farms in Bangladesh.
受污染的家禽和家禽产品是全球人类沙门氏菌病的主要致病源。任何可移动沙门氏菌血清型在家禽中的本地循环都具有更广泛的公共卫生影响，因为它们会通过家禽贸易或人类旅行而传播到其他地方。为了调查孟加拉国种鸡场中可移动沙门氏菌血清型的现状，对两个种鸡场的多批鸡群进行了为期六个月的观察。此外，还进行了横断面调查，通过随机选择 100 个商业肉鸡养殖场，确定可移动沙门氏菌的流行率和血清型分布。采用传统细菌学程序，对代表整个种鸡或肉鸡鸡群的 5 个粪便混合样本进行了可移动沙门氏菌的筛查。随后对获得的沙门氏菌分离株进行血清分型，并通过质粒谱分析和脉冲场凝胶电泳（PFGE）进行特征分析。结果表明，两个种鸡场均为三种沙门氏菌血清型阳性：S. Virchow、S. Paratyphi B var Java（S. Java）和 S. Enteritidis。在所调查的 100 个肉鸡养殖场中，有 11 个养殖场的可移动沙门氏菌呈阳性，农场级别的阳性率为 11%（95%置信区间 5-17%）。从肉鸡养殖场分离出的主要血清型是 S. Virchow 和 S. Kentucky。PFGE 基因分型表明，属于同一血清型的分离株由于仅发生 1-4 条带的变化而密切相关。所有来自种鸡和肉鸡养殖场的 S. Virchow 和 S. Java 分离株均不含质粒，除了一个来自肉鸡养殖场的 S. Virchow 分离株携带一个 9.7 kb 大小的质粒。S. Kentucky 分离株属于三种质粒图谱，具有四种不同大小的质粒，范围从 2.7 到 109 kb。这是首次报告来自孟加拉国种鸡和商业肉鸡养殖场的任何可移动沙门氏菌血清型。
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