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秀丽隐杆线虫幼虫寿命和自噬中的L-异天冬氨酰-O-甲基转移酶

The L-isoaspartyl-O-methyltransferase in Caenorhabditis elegans larval longevity and autophagy.

作者信息

Gomez Tara A, Banfield Kelley L, Trogler Dorothy M, Clarke Steven G

机构信息

Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California-Los Angeles, 607 Charles E. Young Drive East, Los Angeles, CA 90095, USA.

出版信息

Dev Biol. 2007 Mar 15;303(2):493-500. doi: 10.1016/j.ydbio.2006.11.023. Epub 2006 Nov 21.

Abstract

The protein L-isoaspartyl-O-methyltransferase, coded by the pcm-1 gene in Caenorhabditis elegans, participates in the repair of age-damaged proteins. We tested the ability of pcm-1-deficient nematodes to survive starvation stress as developmentally-arrested L1 larvae. We found that pcm-1 mutant L1 larvae do not survive as well as wild-type L1 larvae when incubated in M9 medium without nutrients. We then tested whether the starved L1 larvae could continue development when allowed access to food in a recovery assay. A loss of recovery ability with age was observed for all larvae, with little or no difference between the pcm-1 mutant and wild-type N2 larvae. Interestingly, when L1 larvae were starved in cholesterol-containing S medium or M9 medium supplemented with cholesterol, the survival rates of both mutant and wild-type animals nearly doubles, with pcm-1 larvae again faring more poorly than N2 larvae. Furthermore, L1 larvae cultured in these cholesterol-containing media show an increase in Sudan Black staining over animals cultured in M9 medium. The longevity defects of pcm-1 mutants previously seen in dauer larvae and here in L1 larvae suggest a defect in the ability of pcm-1 mutants to recycle and reuse old cellular components in pathways such as autophagy. Using an autophagosomal marker, we found evidence suggesting that the pcm-1 mutation may inhibit autophagy during dauer formation, suggesting that the absence of protein repair may also interfere with protein degradation pathways.

摘要

由秀丽隐杆线虫中的pcm-1基因编码的蛋白质L-异天冬氨酰-O-甲基转移酶参与衰老损伤蛋白质的修复。我们测试了pcm-1缺陷型线虫作为发育停滞的L1幼虫在饥饿胁迫下的存活能力。我们发现,在不含营养物质的M9培养基中培养时,pcm-1突变型L1幼虫的存活情况不如野生型L1幼虫。然后,我们在恢复试验中测试了饥饿的L1幼虫在获得食物时是否能够继续发育。所有幼虫都观察到随着年龄增长恢复能力的丧失,pcm-1突变型和野生型N2幼虫之间几乎没有差异。有趣的是,当L1幼虫在含胆固醇的S培养基或补充了胆固醇的M9培养基中饥饿时,突变型和野生型动物的存活率几乎都提高了一倍,pcm-1幼虫的情况再次比N2幼虫差得多。此外,在这些含胆固醇的培养基中培养的L1幼虫与在M9培养基中培养的动物相比,苏丹黑染色增加。先前在 dauer 幼虫和此处的L1幼虫中观察到的pcm-1突变体的寿命缺陷表明,pcm-1突变体在自噬等途径中回收和再利用旧细胞成分的能力存在缺陷。使用自噬体标记物,我们发现有证据表明pcm-1突变可能在 dauer 形成过程中抑制自噬,这表明蛋白质修复的缺失也可能干扰蛋白质降解途径。

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本文引用的文献

1
Whole genome RNAi screens for increased longevity: important new insights but not the whole story.
Exp Gerontol. 2006 Oct;41(10):968-73. doi: 10.1016/j.exger.2006.06.048. Epub 2006 Aug 7.
2
Hormonal control of C. elegans dauer formation and life span by a Rieske-like oxygenase.
Dev Cell. 2006 Apr;10(4):473-82. doi: 10.1016/j.devcel.2006.02.008. Epub 2006 Mar 23.
3
Identification of ligands for DAF-12 that govern dauer formation and reproduction in C. elegans.
Cell. 2006 Mar 24;124(6):1209-23. doi: 10.1016/j.cell.2006.01.037. Epub 2006 Mar 9.
4
New genes tied to endocrine, metabolic, and dietary regulation of lifespan from a Caenorhabditis elegans genomic RNAi screen.
PLoS Genet. 2005 Jul;1(1):119-28. doi: 10.1371/journal.pgen.0010017. Epub 2005 Jul 25.
5
A systematic RNAi screen for longevity genes in C. elegans.
Genes Dev. 2005 Jul 1;19(13):1544-55. doi: 10.1101/gad.1308205.
6
Requirement of sterols in the life cycle of the nematode Caenorhabditis elegans.
Semin Cell Dev Biol. 2005 Apr;16(2):175-82. doi: 10.1016/j.semcdb.2005.01.004.
7
Cholesterol-producing transgenic Caenorhabditis elegans lives longer due to newly acquired enhanced stress resistance.
Biochem Biophys Res Commun. 2005 Mar 25;328(4):929-36. doi: 10.1016/j.bbrc.2005.01.050.
8
Methods for monitoring autophagy.
Int J Biochem Cell Biol. 2004 Dec;36(12):2491-502. doi: 10.1016/j.biocel.2004.02.005.
9
Development by self-digestion: molecular mechanisms and biological functions of autophagy.
Dev Cell. 2004 Apr;6(4):463-77. doi: 10.1016/s1534-5807(04)00099-1.

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