Hunter C A, Gow J W, Kennedy P G, Jennings F W, Murray M
Department of Veterinary Medicine, University of Glasgow, United Kingdom.
Infect Immun. 1991 Dec;59(12):4636-40. doi: 10.1128/iai.59.12.4636-4640.1991.
The immunopathology of Trypanosoma brucei brucei in the central nervous system was studied by using an experimental model of chronic meningoencephalitis in outbred CD-1 mice. Mice infected with T. b. brucei were treated with a subcurative dose of the trypanocidal compound diaminazine aceturate. These mice relapsed and were again drug treated. The brains were examined histologically and by immunocytochemistry to identify activated astrocytes. The polymerase chain reaction was used to detect cytokine RNA transcripts. The infected and treated animals developed severe chronic meningoencephalitis characterized by large numbers of inflammatory cells and widespread astrocyte proliferation. In uninfected controls, only interleukin 1 and beta-actin RNA transcripts were detected while transcripts for beta-actin, tumor necrosis factor alpha, macrophage inflammatory protein 1 and interleukins 1 and 4 were demonstrated in the brains of infected animals. Gamma interferon and interleukin 6 were also detected in a few of the infected animals, but interleukin 2 was not found in any of these animals.
通过使用远交群CD-1小鼠慢性脑膜脑炎的实验模型，研究了布氏布氏锥虫在中枢神经系统中的免疫病理学。用亚治疗剂量的杀锥虫化合物乙酰氨基二嗪治疗感染布氏布氏锥虫的小鼠。这些小鼠复发后再次接受药物治疗。通过组织学和免疫细胞化学检查大脑，以鉴定活化的星形胶质细胞。采用聚合酶链反应检测细胞因子RNA转录本。感染并接受治疗的动物发生了严重的慢性脑膜脑炎，其特征为大量炎性细胞和广泛的星形胶质细胞增殖。在未感染的对照中，仅检测到白细胞介素1和β-肌动蛋白RNA转录本，而在感染动物的大脑中证实了β-肌动蛋白、肿瘤坏死因子α、巨噬细胞炎性蛋白1以及白细胞介素1和4的转录本。在少数感染动物中也检测到γ干扰素和白细胞介素6，但在这些动物中均未发现白细胞介素2。
