Liebers V, Schalbaba S, Mazur G, Baur X
Berufsgenossenschaftliches Forschungsinstitut für Arbeitsmedizin, Bochum, FRG.
Int Arch Allergy Appl Immunol. 1991;95(2-3):163-8. doi: 10.1159/000235423.
Hemoglobins (Chi t I) of the dipteron species Chironomus thummi thummi are known to cause severe allergic diseases in humans. We tested the allergen-specific stimulation of human peripheral blood lymphocytes (PBL) by Chi t I and its nine main components. Further, we applied fragments of the well-analyzed component III, obtained by cleavage with trypsin as well as arginine protease. In this way, we screened the molecule in order to identify T-cell epitopes. The whole component was found to be immunogenic and to have regions demonstrating varying PBL stimulation. In addition, interindividual patterns of reactivity, probably due to genetic restriction, were found. A T-cell epitope could be shown to be within the site 98-111, as predicted by application of Rothbard's algorithms.
已知双翅目物种嗜尸摇蚊的血红蛋白（Chit I）会引发人类严重的过敏性疾病。我们检测了Chit I及其九个主要成分对人外周血淋巴细胞（PBL）的变应原特异性刺激。此外，我们应用了经胰蛋白酶和精氨酸蛋白酶切割得到的、已充分分析的成分III的片段。通过这种方式，我们对该分子进行筛选以鉴定T细胞表位。发现整个成分具有免疫原性，且有一些区域显示出不同的PBL刺激作用。此外，还发现了可能由于基因限制导致的个体间反应模式。正如应用罗斯巴德算法所预测的，一个T细胞表位可显示位于98 - 111位点内。
