Liang Chen, Zhang Yu-Rong, Jin Qi-Yun, Guo You-Mei
Shanghai Institute of Criminal Science and Technology, Shanghai 200083, China.
Fa Yi Xue Za Zhi. 2006 Oct 15;22(5):349-52.
To develop a specific, sensitive, reproducible SPE-HPLC method for the determination of 37 drugs in whole blood.
With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood. Two kinds of mobile phases were used in this study. Separations were achieved by a LiChrospher 100 RP-C18 (250 mm x 4.0 mm x 5 microm) column kept at 50 degrees C, the DAD detector was set at 230 nm and 250 nm.
The limit of detection were 1-30 ng/mL. The method showed excellent linearity and the linear correlation coefficient was > or =0.997 98. The relative standard deviation for between-day and within-day assay were <10%.
The method is effective, simple, reliable and has been used in real cases.
