Pasquali Paolo, Nonno Romolo, Mandara Maria Teresa, Di Bari Michele Angelo, Ricci Giovanni, Petrucci Paola, Capuccini Silvia, Cartoni Claudia, Macrì Agostino, Agrimi Umberto
Department of Food Safety and Animal Health, Istituto Superiore di Sanità, viale Regina Elena 299, 00161, Rome, Italy.
BMC Vet Res. 2006 Dec 27;2:37. doi: 10.1186/1746-6148-2-37.
Prion diseases are characterised by a neurodegenerative pattern in which the function of immune system remains still elusive. In the present study, we evaluate if an exogenous treatment with Interleukin-12 (IL-12) and IL-18, able to activate microglia, is able to affect scrapie pathogenesis.
Cytokines injected intracranially, induced a strong inflammatory response characterised by TNF-alpha production and microglia activation. Two groups of mice were injected intracerebrally with high dose of ME7 strain of scrapie containing IL-12 and IL-18 or sterile saline. Cytokines-treated mice showed a more pronounced accumulation of PrPSc in brain tissues at 90 days post-inoculation and a shorter mean survival times than untreated mice.
We can conclude that intracerebral administration of IL-12 and IL-18 can modulate scrapie pathogenesis possibly through a microglia-mediated pattern.
朊病毒疾病的特征是神经退行性病变模式，其中免疫系统的功能仍然难以捉摸。在本研究中，我们评估了外源性给予白细胞介素-12（IL-12）和IL-18（能够激活小胶质细胞）是否能够影响羊瘙痒病的发病机制。
颅内注射细胞因子诱导了以肿瘤坏死因子-α产生和小胶质细胞激活为特征的强烈炎症反应。两组小鼠分别脑内注射含有IL-12和IL-18的高剂量羊瘙痒病ME7毒株或无菌生理盐水。细胞因子处理的小鼠在接种后90天脑组织中PrPSc的积累更为明显，平均存活时间比未处理的小鼠短。
我们可以得出结论，脑内给予IL-12和IL-18可能通过小胶质细胞介导的模式调节羊瘙痒病的发病机制。
