Melani Raffaella, Sallustio Fabio, Fardin Paolo, Vanni Cristina, Ognibene Marzia, Ottaviano Catherine, Melillo Giovanni, Varesio Luigi, Eva Alessandra
Laboratorio di Biologia Molecolare, Istituto G. Gaslini, Largo G. Gaslini 5, 16147 Genova, Italy.
Gene Expr. 2006;13(3):155-65. doi: 10.3727/000000006783991845.
The Dbl oncogene is a guanine nucleotide exchange factor for Rho GTPases and its activity has been linked to the regulation of gene transcription. Dbl oncogene expression in NIH3T3 cells leads to changes in morphological and proliferative properties of these cells, inducing a highly transformed phenotype. To gain insights into Dbl oncogene-induced transformation we compared gene expression profiles between Dbl oncogene-transformed and parental NIH3T3 cells by cDNA microarray. We found that Dbl oncogene expression is associated with gene expression modulation involving upregulation of 51 genes and downregulation of 49 genes. Five of the overexpressed genes identified are known to exert antiproliferative functions. Our observations suggest that the expression of Dbl oncogene in NIH3T3 may lead to the induction of genes associated with cell cycle arrest, possibly through the activation of stress-induced kinases.
Dbl癌基因是一种Rho GTP酶的鸟嘌呤核苷酸交换因子,其活性与基因转录调控有关。NIH3T3细胞中Dbl癌基因的表达会导致这些细胞的形态和增殖特性发生变化,诱导出高度转化的表型。为深入了解Dbl癌基因诱导的转化过程,我们通过cDNA微阵列比较了Dbl癌基因转化的NIH3T3细胞和亲本NIH3T3细胞之间的基因表达谱。我们发现,Dbl癌基因的表达与基因表达调节有关,其中51个基因上调,49个基因下调。已确定的5个过表达基因具有抗增殖功能。我们的观察结果表明,NIH3T3细胞中Dbl癌基因的表达可能通过激活应激诱导激酶,导致与细胞周期停滞相关的基因被诱导。