Albertini S, Zimmermann F K
Department of Toxicology, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
Mutat Res. 1991 Nov;258(3):237-58. doi: 10.1016/0165-1110(91)90011-j.
Our objective is to summarize the published data obtained with a recently developed tester strain suitable for the detection of chromosomal malsegregation in yeast. Results from 25 papers were reviewed in which numerical data for 111 chemicals tested in Saccharomyces cerevisiae D61.M are reported (a total of 316 independent tests; 279 acceptable, 37 not meeting our criteria). Of the 111 compounds analyzed 43 compounds are positive for chromosomal malsegregation, 56 compounds are negative and 12 compounds do not meet our criteria for acceptance (inconclusive). Of the 43 compounds judged positive 5 (acetone, acetonitrile, benzonitrile, ethylacetate and propionitrile) were only positive using a cold interruption protocol. Recommendations are made for standardization of methods and protocols for screening purposes. Finally, a comparison with in vitro tubulin assembly data using mammalian tubulin is presented.
我们的目标是总结使用最近开发的适合检测酵母中染色体错分离的测试菌株所获得的已发表数据。回顾了25篇论文的结果，其中报告了在酿酒酵母D61.M中测试的111种化学物质的数值数据（总共316次独立测试；279次可接受，37次不符合我们的标准）。在分析的111种化合物中，43种化合物染色体错分离呈阳性，56种化合物呈阴性，12种化合物不符合我们的接受标准（结果不确定）。在判定为阳性的43种化合物中，有5种（丙酮、乙腈、苄腈、乙酸乙酯和丙腈）仅在冷中断方案下呈阳性。针对筛选目的的方法和方案标准化提出了建议。最后，给出了与使用哺乳动物微管蛋白的体外微管蛋白组装数据的比较。
