Lillie Brandon N, Keirstead Natalie D, Squires E James, Hayes M Anthony
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ont., Canada N1G 2W1.
Dev Comp Immunol. 2007;31(8):830-46. doi: 10.1016/j.dci.2006.11.002. Epub 2006 Dec 11.
Previous studies showed that low expression of mannan-binding lectin C (MBL-C) in pigs was not due to single-nucleotide polymorphisms (SNPs) in the coding region of pig MBL2. In these studies, we compared the 5' flanking regions of porcine MBL1 (1907 bp) and MBL2 (1880 bp) in normal and diseased pigs with low or high hepatic expression of MBL2. Hepatic expression of MBL-C was very low in all pigs submitted for postmortem diagnosis. In various European pig breeds, a G(-1081)A substitution was linked to very low hepatic MBL-C expression, and was more frequent in diseased pigs. A C(-251)T substitution with less influence on MBL-C expression was more common in various breeds but was not associated with disease. MBL2 polymorphisms were associated with some disease groups and with the presence of some etiologic agents. These findings indicate that some promoter polymorphisms impair MBL-C expression in pigs and may increase their susceptibility to disease.
先前的研究表明，猪体内甘露聚糖结合凝集素C（MBL-C）的低表达并非由于猪MBL2编码区的单核苷酸多态性（SNP）所致。在这些研究中，我们比较了正常猪和患病猪（MBL2肝脏表达水平低或高）中猪MBL1（1907 bp）和MBL2（1880 bp）的5'侧翼区域。所有用于尸检诊断的猪中，MBL-C的肝脏表达都非常低。在各种欧洲猪品种中，G（-1081）A替换与肝脏MBL-C的极低表达有关，并且在患病猪中更常见。对MBL-C表达影响较小的C（-251）T替换在各种品种中更常见，但与疾病无关。MBL2多态性与一些疾病组以及一些病原体的存在有关。这些发现表明，一些启动子多态性会损害猪体内MBL-C的表达，并可能增加它们对疾病的易感性。
