Tavolaro Adalgisa, Tavolaro Palmira, Drioli Enrico
Research Institute on Membrane Technology, ITM-CNR, Via Bucci Cubo 17/c, University of Calabria, I-87030 Arcavacata di Rende, Italy.
Colloids Surf B Biointerfaces. 2007 Mar 15;55(1):67-76. doi: 10.1016/j.colsurfb.2006.11.010. Epub 2006 Nov 17.
Zeolites due to their low toxicity and high compatibility are considered new biomaterials for medical applications. The surface adsorption behaviour of zeolite crystals and composite membranes was discussed in this research. The zeolite materials were synthesized by hydrothermal syntheses using different reaction gels to modulate the Brönsted acidity of the microporous structures. Spectrophotometric analyses were used to evaluate protein adsorption on these surfaces. This study revealed that zeolite chemical composition and structure influenced the kinetics of protein adsorption. Zeolite Y surface adsorbed greater amount of BSA than the other structures. The percentage of adsorption increases with temperature and depends on the pH of the solution, being highest at the pI of the protein. The influence of the membrane configuration on the protein adsorption was studied using different zeolite structures and crystallization types. It seems that the observed differences could depend on the type of hydrothermal crystallization inside the inorganic support.
由于沸石具有低毒性和高兼容性，它们被认为是用于医学应用的新型生物材料。本研究讨论了沸石晶体和复合膜的表面吸附行为。通过水热合成法，使用不同的反应凝胶来调节微孔结构的布朗斯特酸度，从而合成沸石材料。采用分光光度分析来评估蛋白质在这些表面上的吸附情况。该研究表明，沸石的化学成分和结构影响蛋白质吸附的动力学。Y型沸石表面比其他结构吸附的牛血清白蛋白（BSA）量更多。吸附百分比随温度升高而增加，并且取决于溶液的pH值，在蛋白质的等电点（pI）时最高。使用不同的沸石结构和结晶类型研究了膜构型对蛋白质吸附的影响。观察到的差异似乎可能取决于无机载体内部水热结晶的类型。
