一种基于吸附在硅沸石纳米颗粒上的重组脲酶的具有改进分析特性的新型电导式尿素生物传感器。
A Novel Conductometric Urea Biosensor with Improved Analytical Characteristic Based on Recombinant Urease Adsorbed on Nanoparticle of Silicalite.
作者信息
Velychko T P, Soldatkin О О, Melnyk V G, Marchenko S V, Kirdeciler S K, Akata B, Soldatkin A P, El'skaya A V, Dzyadevych S V
机构信息
Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, 03143, Kyiv, Ukraine.
Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01003, Kyiv, Ukraine.
出版信息
Nanoscale Res Lett. 2016 Dec;11(1):106. doi: 10.1186/s11671-016-1310-3. Epub 2016 Feb 25.
Development of a conductometric biosensor for the urea detection has been reported. It was created using a non-typical method of the recombinant urease immobilization via adsorption on nanoporous particles of silicalite. It should be noted that this biosensor has a number of advantages, such as simple and fast performance, the absence of toxic compounds during biosensor preparation, and high reproducibility (RSD = 5.1 %). The linear range of urea determination by using the biosensor was 0.05-15 mM, and a lower limit of urea detection was 20 μM. The bioselective element was found to be stable for 19 days. The characteristics of recombinant urease-based biomembranes, such as dependence of responses on the protein and ion concentrations, were investigated. It is shown that the developed biosensor can be successfully used for the urea analysis during renal dialysis.
已有报道开发出一种用于尿素检测的电导式生物传感器。它是通过一种非典型方法制备的，即将重组脲酶吸附在硅沸石纳米多孔颗粒上进行固定化。需要注意的是，这种生物传感器具有许多优点，如性能简单快速、生物传感器制备过程中无有毒化合物以及重现性高（相对标准偏差RSD = 5.1%）。使用该生物传感器测定尿素的线性范围为0.05 - 15 mM，尿素检测下限为20 μM。发现生物选择性元件可稳定19天。研究了基于重组脲酶的生物膜的特性，如响应与蛋白质和离子浓度的关系。结果表明，所开发的生物传感器可成功用于肾透析过程中的尿素分析。
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