Gamian A, Krzyzaniak A, Barciszewska M Z, Gawrońska I, Barciszewski J
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan.
Nucleic Acids Res. 1991 Nov 11;19(21):6021-5. doi: 10.1093/nar/19.21.6021.
It has been found that the bacterial endotoxins (lipopolysaccharides, LPSs) contain some amino acids and glycine is the most abundant amino acid in the polysaccharide core preparations of LPSs of gram-negative bacteria. Until now nothing was known about the mechanism of amino acid incorporation into the lipopolysaccharide core. We found that one out of three glycyl-tRNAs(Gly) from Escherichia coli is the donor of amino acid and is the substrate for a putative aminoacyl-tRNA:LPS transferase. We have isolated, purified this tRNA and determined its nucleotide sequence to be major E.coli tRNA(3Gly). This tRNA(Gly) (anticodon GCC) conserved the tRNA structural features. The assay for determination of the specific incorporation of glycine into the lipopolysaccharide was also invented and described.
已发现细菌内毒素（脂多糖，LPSs）含有一些氨基酸，且甘氨酸是革兰氏阴性菌LPSs多糖核心制剂中含量最丰富的氨基酸。到目前为止，关于氨基酸掺入脂多糖核心的机制尚不清楚。我们发现，来自大肠杆菌的三种甘氨酰 - tRNA（Gly）中有一种是氨基酸供体，并且是一种假定的氨酰 - tRNA：LPS转移酶的底物。我们已经分离、纯化了这种tRNA，并确定其核苷酸序列为主要的大肠杆菌tRNA（3Gly）。这种tRNA（Gly）（反密码子GCC）保留了tRNA的结构特征。还发明并描述了用于测定甘氨酸特异性掺入脂多糖的方法。
