McClain W H, Foss K, Jenkins R A, Schneider J
Department of Bacteriology, University of Wisconsin, Madison 53706-1567.
Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6147-51. doi: 10.1073/pnas.88.14.6147.
Expression of the genetic code depends on the recognition of specific tRNAs by the enzymes that aminoacylate them. A computer comparison of tRNA sequences coupled with analysis of mutant nonsense-suppressor tRNAs has revealed the structural features that distinguish the acceptor identity of Escherichia coli tRNA(Gly) from tRNAs that accept phenylalanine, arginine, lysine, and glutamine. On replacement of several nucleotides in the acceptor stem and anticodon of the latter tRNAs with tRNA(Gly)-derived residues, the resulting molecules acquired a tRNA(Gly) identity.
遗传密码的表达取决于使tRNA氨酰化的酶对特定tRNA的识别。tRNA序列的计算机比较以及对突变的无义抑制tRNA的分析揭示了区分大肠杆菌tRNA（Gly）与接受苯丙氨酸、精氨酸、赖氨酸和谷氨酰胺的tRNA的受体身份的结构特征。用tRNA（Gly）衍生的残基替换后一种tRNA的受体茎和反密码子中的几个核苷酸，得到的分子获得了tRNA（Gly）的身份。
